The definition of the Isoelectric point (pI) of a protein.

What is the definition of the "isoelectric point" (pI) of a protein?

The calculation of the isoelectric point (pI) of an amino acid. General method, step by step solutions to the problems, and definitions of concepts/terms provided.

Calculate the isoelectric point (pI) of each of the following amino acids (a) glycine (b) glutamic acid (c) arginine

Understanding and using the Henri-Michaelis-Menten equation.

For an enzyme which obeys Henri-Michaelis-Menten kinetics, (1) At what substrate concentration will an enzyme characterized by a kcat of 30 s-1 and Km of 0.005 M show one quarter of its maximal rate? (2) Calculate the fraction of Vmax that would be found when [S]=0.5 Km, [S]=3Km, and [S]=10Km

Understanding under what conditions an enzyme achieves maximal velocity (Vmax) and the turnover number of an enzyme.

The enzyme carbonic anhydrase is found in erythrocytes, and has a molecular mass of approximately 30000 Da. The hydration reaction catalyzed by this enzyme is reversible, and plays a role in the transport of CO2 from the tissues to the lungs. H2O + CO2 <-> H2CO3 If 20 micrograms of pure enzyme catalyzes the hydration of ...continues

Enzyme Kinetics: Understanding the Henri-Michaelis-Menten equation/model.

An enzyme with a Km of 0.36 mM was assayed using substrate concentrations of 3 x 10E-7 M, 9.5 x 10E-5 M, 1.5 x 10E-4, 1.16x10E-3, 3 x 10E-3 M, 0.01 M, 0.08 M. At a substrate concentration of 0.08 M, the initial velocity was 192 nM/min. Calculate the initial velocity at each of the other substrate concentrations. Graph the cal ...continues

Determining the reaction direction, initial velocity of reaction and the Haldane relationship in enzyme kinetics.

A reversible reaction described by the chemical equation shown below (equation one) has an equilibrium constant of 10. (S represents substrate, P represents product.) If we have a mixture containing [S]=2x10E-5 M and [P]=3x10E-5M, and we know that KmS=3x10E-5 M, Vmax (forward reaction) =2 micromole /(L min), and Vmax (reverse ...continues

Literature review of the conversion of RNA into DNA.

With particular emphasis on the biochemistry, discuss the machinery involved in the transcriptional process.

Determining the pKa of an acid.

For a weak electrolyte that ionizes as: HX --> H+ + X- construct a buffer with a ratio of 5 moles of HX/2.1 moles X-. The reaction is maintained at pH = 8.7. What is the pKa of the acid?

This is an example of how to do an indepth analysis of a research article. This analysis is on mRNA degradation.

Analyze a peer-reviewed journal article having to do with biochemical research into mRNA degradation.

Molecular Biology Homework Help and Solution

Please see the attached file for full problem description. --- 1. Heparin is a polyanion that inhibits RNA transcription in vitro by binding directly to bacterial RNA polymerase (RNAP). If heparin interferes with the ability of the polymerase to bind DNA in a non-specific fashion, what subunit of RNAP is the heparin binding ...continues