calculating the isoelectric point of a protein - I'm trying to calculate the isoelectric point of a protein. I understand that you need to substitute variations of pH until you find a pI that is close to zero. What I need is a detailed example of th ...
GRE Biochem question - The galactose represser protein from E. coli has a pI of about 5.9. While purification protocols were being designed, it was found to bind to a Mono-S column at pH values of 7 and below. (Mono-S colum ...
pH of glycine after addition of acid/base - If I had 1L of a 1.0M solution of glycine at the isoelectric pH (6.02) and I added 0.3 mol of HCL, what would be the new pH? What would be the pH if I added 0.3 mol of NaOH instead of the acid?
my t ...
GRE Subject Biochem problem (Chromatography Elution) -
Sketch the expected chromatographic elution profile (protein concentration vs elution volume) if a mixture of these proteins is applied to a weak anion exchange resin such as DEAE-cellulose at ...
pI (isoelectric poin) - based on predicted pI (for example, a pI for turnip peroxidase is 9.10), how can you use DEAE-sepharose to further purify peroxidase?