Biochemistry Homework Solutions
#1768
Understanding and using the Henri-Michaelis-Menten equation.
For an enzyme which obeys Henri-Michaelis-Menten kinetics,
(1) At what substrate concentration will an enzyme characterized by a kcat of 30 s-1 and Km of 0.005 M show one quarter of its maximal rate?
(2) Calculate the fraction of Vmax that would be found when [S]=0.5 Km, [S]=3Km, and [S]=10Km
This solution shows an example of how to calculate common enzyme kinetic parameters (kcat, Km, Vmax, etc.), using the Henri-Michaelis-Menten equation. A step by step solution is provided.
What is this?
By OTA - Overall OTA Rating
Monica Tory, MSc - 3.8/5
Purchase Cost Now
$2.19 CAD
Included in Download
- Plain text response
- Attached file(s):
- kinetics.doc
Why you can trust BrainMass.com
- Your Information is Secure
- Best Online Academic Help Service
- Students find real academic Success
- Relation between reaction velocity and substrate concentration:Michaelis-Menten Equation - Can someone please go through the steps to help me get the correct solutions? Thank you. 1) At what substrate concentration would an enzyme show 30% of its maximum catalytic rate if the Km of the ...
- Biochem- KM of a Michaelis-Menten enzyme - 5. The KM of a Michaelis-Menten enzyme for a substrate is 1.0 x 10-4 M. At a substrate concentration of 0.2 M, νo = 43 μM x min-1 for a certain enzyme concentration. However, with a substrat ...
- The rate equation for an enzyme subject to competitive inhibition - The rate equation for an enzyme subject to competitive inhibition is given below. Vo = Vmax[S]/(KM + [S]) Where = 1 + ([I]/KI) and the dissociation constant KI= [E] ...
- Michaelis-Menten Reactions - Please explain: An enzyme catalyzes a reaction at 20μmol/min when the substrate [s] is 0.01M. The Km for this substrate is 1 x 10-5M Assuming michaelis-menten kinetics are followed, what would t ...
- Michaelis-Menten Method; Effect of pH on Enzyme - Phosphofructokinase is an enzyme, widespread in nature, catalyzing the reaction: fructose-6-phosphate + ATP ----------> fructose-1,6-diphospohate + ADP Under conditions where the concentration o ...
