Biochemistry Homework Solutions
Problem
#2282

Determining the reaction direction, initial velocity of reaction and the Haldane relationship in enzyme kinetics.

A reversible reaction described by the chemical equation shown below (equation one) has an equilibrium constant of 10.  (S represents substrate, P represents product.)  If we have a mixture containing [S]=2x10E-5 M and [P]=3x10E-5M, and we know that KmS=3x10E-5 M, Vmax (forward reaction) =2 micromole /(L min), and Vmax (reverse reaction) = 4 micromole /(L min), answer the following questions.

(a) In which direction will the reaction proceed when the appropriate enzyme is added?
(b) After addition of enzyme, what will the initial velocity be when the reaction starts toward equilibrium?

Equation one:

(refer to the attached file for the equation)

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enzymeQ.doc
A reversible reaction described by the chemical equation shown below
(equation one) has an equilibrium constant of 10. (S represents
substrate, P represents product.) If we have a mixture containing
[S]=2x10E-5 M and [P]=3x10E-5M, and we know that KmS=3x10E-5 M, Vmax
(forward reaction) =2 micromole /(L min), and Vmax (reverse reaction) =
4 micromole /(L min), answer the following questions.

In which direction will the reaction proceed when the appropriate enzyme
is added?

After addition of enzyme, what will the initial velocity be when the
reaction starts toward equilibrium?

Equation one:


Solution
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