A cake is advertised to be fat-free.Hydrolysis of cake shows fructose

A cake is advertised to be fat-free. Hydrolysis of cake mix yeilds glocose, fructose, glycerol, a number of amino acides and molecules with long hydrocarbon chains-most of these chains have a carboxyl group at the end. What do you report to the FDA?

Citric Acid Cycle

citrate ---> isocitrate delta G = +13.3 kJ/mol In the citric acid cycle, the conversion of citrate to isocitrate doesn't seem to require ATP or another high energy compound. From a *quantitative* standpoint, what are the circumstances that allow this reaction to proceed? How does the cell overcome the pos. delta ...continues

mRNA

Why is mRNA so unstable in most bacteria with a halflife of about 1min compared to many higher organisms where the halflife is much longer?

Tripeptides

Tripeptide1: Tyr-Lys-Met Tripeptide2: Gly-Pro-Arg Tripeptide3: Asp-Trp-Tyr Tripeptide4: Asp-His-Glu Tripeptide5: Leu-Val-Phe Which one of the above tripeptides: a. is most negatively charged at pH7 b. contains the largest number of nonpolar R groups c. contains sulfur d. will have the greatest light absorbance at 280 ...continues

Net electrical charge

Calculate the net electrical charge for the below polypeptide sequence at pH 7.4 Glycinie-Lysine-Glutamate-Arginine-Histidine-Tyrosine

Growth rate

Calculate the maximum growth rate (umax) and the specific growth rate (u) at t=10h Time (h), X(g/L) Cell Growth 0, 0.2 2, 0.211 4, 0.305 8, 0.98 10, 1.77 12, 3.2 14, 5.6 16, 6.15 18, 6.2 dX/dt = unetX, when u is constant lnX-lnX0 = ut Attached word doc for more clarity

Using the data given in table below plot a Lineweaver-Burk plot

a) Using the data given in table below plot a Lineweaver-Burk plot b) Determine the Km and Vmax for the three sets of data c) Which inhibitor would the most effective at high substrate concentrations? d) Explain you choice Table 1 : Kinetics data for an enzyme catalysed reaction with and without the presence of ...continues

What is tertiary structure of proteins and how is the tertiary structure generally stabilized?

What is tertiary structure of proteins and how is the tertiary structure generally stabilized?

universal priming sequences and sequencing cloned cDNA

A 2500 base-pair long cDNA of unknown sequence was cloned into the MCS of a plasmid vector that has universal priming sequences adjacent to and on both sides of the MCS. On one side this is called the "forward universal priming sequence" and on the other it is called the "reverse universal priming sequence." Sanger sequencing ...continues

pH, pka & ionization

I need help with the following correlation: In the SIMPLEST terms possible, please describe how one would use the concepts of pH and pka to predict the ionization state of any given amino acid or its side chain in a protein. Thank you very much :)