Biochemistry Homework Solutions

DNA composition

A sample of DNA purified from Mycobacterium tuberculosis contains 15.1% Adenine on a molar basis. What are the percentages of the other bases present?

Relation between reaction velocity and substrate concentration:Michaelis-Menten Equation

Can someone please go through the steps to help me get the correct solutions? Thank you. 1) At what substrate concentration would an enzyme show 30% of its maximum catalytic rate if the Km of the enzyme is 0.05 mM? Answer: 0.021 mM 2) At what substrate concentration would an enzyme show one-quarter of its maximum rate ...continues

Peroxidase

What are peroxidase? - Include a brief description of the general type of reaction they catalyze. - Describe a metabolic pathway from any organism (baterium, plant, animal) that peroxidase is involved. - Include the name of the specific peroxidase, the components of the reaction catalyzed by the peroxidase and the name o ...continues

Bradford Assay

What are the possible errors when the Bradford assay is used for the protein determination?

pI (isoelectric poin)

based on predicted pI (for example, a pI for turnip peroxidase is 9.10), how can you use DEAE-sepharose to further purify peroxidase?

pH buffer peroxidase

pI for peroxidase is 9.10 what pH of buffers would allow for the binding and elution of the peroxidase from the resin?

Enzyme

1. What does rate enhancement means? (in simplest term and in detail please ) 2. describe the energetic of catalysis in simplest terms? 3. How do enzymes affect forward and reverse reaction rate & what does this means?

Chymostrypsin

How do I sketch the active site, including the catalytic triad? How do these three residue act to allow proteolysis? Can someone briefly explain to me the features of the chymostrypsin active site and the mechanism?

Cortisol

What gland produces cortisol? What factors regulate cortisol production? What functions are controlled by cortisol?

Enzyme Inhibition

Lineweaver-Burk Plot, Lineweaver-Burk equation and plot of graph. Competitive inhibition and uncompetitive inhibition.

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