What properties of membranes are responsible for this important feature?
(J) Cellular membranes are self-sealing if they are punctured or mechanically disrupted. They quickly and automatically reseal. What properties of membranes are responsible for this important feature?
(H) Although proteins rarely (if ever) flip-flop across a membrane, the distribution of membrane lipids between the membrane leaflets is not absolute, except for glycolipids. Please explain why glycosylated lipids are less likely to flip-flop.
(C) The first protein structure to be determined was myoglobin from sperm whale. Please propose an explanation for the observation that sperm whale muscle is rich in this protein.
(D) Under appropriate conditions, hemoglobin dissociates into four subunits. The isolated alpha subunit binds oxygen, but the O2-saturation curve is hyperbolic rather than sigmoidal. In addition, the binding of oxygen to the isolated alpha subunit is not affected by H+, CO2, or BPG. What do these observations indicate about t ...continues
(G) A common structural feature of membrane lipids is their amphipathic nature. For each of the following lipids, name the components that serve as hydrophobic and hydrophilic units: (1) phosphatidylethanolamine (2) sphingomyelin (3) galactosylcerebroside (4) ganglioside (5) cholesterol
Gene identification, RNA probe
Design (an) RNA probe(s) with 18-20 nucleotides that will allow you to identify by hybridization the gene for a protein with the following N-terminal amino acid sequence. H3N+- ala-pro-met-thr-trp-tyr-cys-met-asp-trp-ile-ala-gly-gly-pro-trp-phe-arg-lys-asn-thr-lys-. Explain your reasoning.
Protein structure and function
In the following polypeptide chain where do you expect to see bends, twists, and disulfide linkages forming? Which ones of these amino acids is/are likely to be in the interior of a protein? Mark the amino acids likely to contribute to the obverall charge of the peptide in neutral pH. Ile-Ala-His-Thr-Tyr-Gly-Pro-Phe-Glu-Ala ...continues
Please write a script AND some presentation powepoint slides for the attached article...Preparation of enzymatically active recombinant The script have to include: Background info, reference to related articles, explaining significance of the article, Explanation of the actual content of the article.
Compare competitive and noncompetitive in terms of A) how Km is effected in presence of inhibitor, B) how Vmax is effected in presence of inhibitor, C) where inhibitor would bind to the enzyme
The advantage of treating separate samples of a protein with two or more enzymes when sequencing a protein is that the products are a. more homogeneous b. sequenceable with out further chromatography c. fragments with the same N- and C-terminal amino acids d. fragments with sequence overlaps e. all are true