Biochemistry Lab - Assistance in writing "introduction" and "experimental procedure". Please follow the guideline (file1 and 2) and manual_3 file. See attached file for full problem description.
The rate equation for an enzyme subject to competitive inhibition
The rate equation for an enzyme subject to competitive inhibition is given below. Vo = Vmax[S]/(KM + [S]) Where = 1 + ([I]/KI) and the dissociation constant KI= [E][S]/[EI] Beginning with the total enzyme [ET]= [E] + [EI] + [ES], please derive the rate equation above. (Hint) Use the deri ...continues
What is the energy cost of this kind of covalent regulation?
The phosphorylation and dephosphorylation of proteins is a vital means of regulation. Protein kinases attach phosphoryl groups, whereas phosphatases remove the phosphoryl group from the target protein. What is the energy cost of this kind of covalent regulation?
How do I figure out the ionic structures of glutathione? And how do I figure out the net charge when in the blood at ph 7.4?
Are yeasts better anaerobic or aerobic? Why?
Why does fluoracetate cause citrate to build up in the Citric Acid Cycle? Why do glucose and glycolysis decrease and hexose increases?Why is it fatal? What about fluoroacetyl-SCoA instead in the CAC? Why is it blocking or inhibiting?
What are the possible steps that would convert glycerol-1-phosphate to ethanol and carbon dioxide?
Is D-mannose or D-lyxose a better substrate for D-mannose isomerase and why? Would a L form also be an substrate?
Why would insulin like Humulin and Lantus be ineffective if taken orally? How do the structures affect the action rate? how do nonpolar anesthetics disrupt nerve impulses?What products are produced by ingestion of a retrovirus like AIDs? Why is catabolism of fatty and amino acids required to be aerobic? Why is pyruvate carboxyla ...continues
Enzyme-catalysed reaction: activation energy
Here is the problem I have to solve: If 10 μg of pure carbonic anhydrase catalyzes the hydration of 0.30 g of carbon dioxide in 1 min at 37˚C under optimal conditions, what is the turnover number (kcat) of carbonic anhydrase (in units of min-1)? I have already calculated the turnover number, which is 2.0 x 107 min ...continues
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