Pulse Chase Experiment

If glucose-1 is the radioactive source, where is 14C in ethanol product? Anaerobic conditions. where would it be if the 14C would be liberated as CO2 during fermentation?

Blood Type

How do blood group antigens differ between O, A, and B. Is it the Gal alpha 1 kind of thing? How could different antigens be produced in cells? only a real simple explanation is needed.

Insulin question

I need to know how the specific structures of Humulin and Lantus are consistent with whether they act quickly or slowly. I know humulin is recombinant DNA and acts quickly and Lantus is proinsulin and slow but just need really simple explanation why.

Glycogen synthesis and glycogen degradation

Metabolism uses various ways to ensure that 2 opposing pathways do not occur at the same time. For the following pair of pathways list 2 ways (different cell location, allosteric effector, covalent modification, different intermediate, different enzyme, or different means of transport). How are glycogen synthesis and glycog ...continues

Citric acid and urea cylce amino acids

Which 2 amino acids donate amino groups to other compounds for nitrogen metabolism? What citric acid cycle intermediate can be converted to aspartic acid upon acceptance of an amino group? What coenzyme shared by pentose phosphate pathway and by lipid biosynthesis is also used to make glutamate? What citric acid cycle inte ...continues

Controlled Experiment Design

Describe a well-controlled experiment to determine if the rise in internal free calcium observed in the cytoplasm of unfertilized sea urchin eggs in the presence of A23187 is due to in flux of calcium from seawater. Include experimental treatments, controls, and briefly describe experimental methods. Note: As mentioned in th ...continues

SDS-PAGE

What is the importance of acrylamide concentration in SDS-PAGE?

Biochemical Techniques

What are the possible source of errors for agarose gel? What are the possible source of errors for using spectrophotometer for reading absorbence at 260 nm? I did a lab where I had to find the concentration and yield of fluorescent protein plasmid DNA (label as "FP3"). The results I obtained: DNA Concentration of FP3 (ug ...continues

Biochemical Techniques (2)

Two fluorescent protein plasmid DNA was digest with restriction enzyme: BamH1. These two fluorescent protein plasmid DNA was labeled: FP3 and FP6. (These two are different fluorescent protein). I calculated the theoretical yield for FP3 and obtained a value of 14.5 ug. Would you expect it to be the same or different for the othe ...continues

Biochemical Techniques

"Restriction digests are done for a number of reasons such as verifying that a clone is correct or constructing a restriction map of a plasmid you were given. From your digests you should be able to determine the size of the gene encoding the FPs you were given. For one of your clones, use the size of the gene to estimate the MW ...continues