Tripalmitoylglycerol Oxidation

Contrary to the legend camels do not store water in their humps, which actually consist of a large fat deposit. How can these fat deposits serve as a source of water? Calculate the amount of water (in liters) that can be produced by the camel from 1 kg (0.45 lb) of fat. Assume for simplicity that the fat consists entirely ...continues

Amino acids and nitrogen products

Although normal E.coli cells can synthesize all the amino acids, some mutants called amino acid auxotrophs, are unable to synthesize specific amino acids and require the addition of that amino acid to the culture medium for optimal growth. In addition to their role in protein synthesis, specific amino acids are also requir ...continues

Effect on PAGE gel

Describe the effects the following would have on the way you PAGE gel would run: a) Increasing the percent of acrylamide b) Increasing the pH of the Tris-glycine buffer to 9.5 c) Decreasing the voltage at which the gel was run d) Increasing the voltage at which the gel was run by a factor of 3 e) Doubling the sample load vo ...continues

Making a dilution

Make the following dilution: a working concentration of 100 ml 15 U/uL from an enzyme stock of 756,431 U/uL. Will you use a serial dilution? Why or Why not?

DNA

1. Describe DNA sequencing with reference to the cloning of a DNA fragment in a bacteriophage M13 based cloning vector. 2. Describe the use of a expression vector to isolate the gene from a cDNA library that codes for a specific protein.

Genetics

5. Explain what proto-oncogenes are and describe their role as intracellular signal transducers and transcription factors. 6. Explain the notion that cancer is seen a genetic disease and that the predisposition of cancer can be inherited.

Cloned Genes/Genetic Disease

8. Explain how information of the sequence of cloned genes can shed light on the function of their coding genes. 9. Explain how PCR can be used in the diagnosis of a genetic disease.

Activity Assay & pH Problems

I am carrying out an activity assay on beta-glucosidase from almonds using 4-nitrophenol. In order for the 4-nitrophenol to release the yellow colour expected, it has to be fully ionised. With a pKa of 7, I have decided that pH9 would allow it to be fully ionised - this is also supported by the yellow colour given off, and a ...continues

Beta Glucosidase Activity Assay

I have followed the procedure that you provided to determine the activity of the beta glucosidase, however what I am unsure of is how the rate is calculated? In order for me to establish the rate, I followed your instructions however incubated the enzyme/buffer and PNPG solution for different times, ranging from 0-16 min ...continues

Calculating the pKa of a weak acid

Step-by-step explanation of how to calculate the pKa of a weak acid of known pH and concentration.