Proton concentration

If a 250 ml solution of 125mM acetic acid is titrated with sodium hydroxide to pH=3.93. What is the proton concentration? pKa of acetic acid is 4.76

Phosphate system serves to buffer the intracellular fluid of cells at physiological pH

The phosphate system serves to buffer the intracellular fluid of cells at physiological pH because pK2 (7.20) lies near the normal pH range of 6.9-7.4. If the total cellular concentration of phosphate is 19mM and the pH is 6.9, what is the concentration of (H2PO4-) in mM?? Is this correct??? H2PO4-(aq) H+(aq) + HPO42-(aq) ...continues

Delta G

The equilibrium constant for the denaturation of a protein is determined to be 0.13 at 59.8 degrees celsius. What is the standard state of delta G for the reaction, in kJ/mol to 0.01kJ/mol.

Hydrolysis of Phosphocreatine

For the hydrolysis of phosphocreatine: Phosphocreatine + H2O==creatine + Pi the standard state deltaG is -42.8 kJ/mol at 37 degrees celsius. If in a given cell the concentration of creatine is 1.7mM, phosphate is 5.8mM, and phosphocreatine is 6.4mM, what is the deltaG for hydrolysis of phosphocreatine in the cell? nearest 0. ...continues

Two problems in enzyme kinetics invloving kcat and ki.

1. A new enzyme you are studying has a Km of 7.0mM and a kcat of 126 per second. What will be the initial rate of reaction in units mM/min when 9.3 nanometer enzyme is reacted with 2.7 mM substrate. Report your answer in units if mM/min to nearest 1mM/min? 2. A new enzyme you are studying has a Km of 12.6 mM and a kcat o ...continues

Discuss the methods used to separate hexokinases A, B and C.

I am a bit confused on the purification procedures (attached) for hexokinase B and C, and would like someone to give an explanation on the steps for both of these procedures, as the paper they are in does not really include a lucid explanation. Also, why is there a difference in the purification of hexokinase B and hexokinase C ...continues

Non-competitive inhibitors of enzymes change the slope and Km of a Lineweaver Burk graph compared to no inhibitor. a.) True b.) False

Non-competitive inhibitors of enzymes change the slope and Km of a Lineweaver Burk graph compared to no inhibitor. a.) True b.) False

Multiple Choice - Which level of structure must a protein have to be allosterically regulated?

I have attached the five questions and my proposed answers. Please look over them and if they are incorrect, please correct them. I appreciate the help. 1. Since allosteric proteins change shape, in order to perform other biological functions and bind with cofactors, I would say the primary level of protein structure, answer ...continues

Free Energy of Fructose-6-Phosphate and Glucose-6-Phosphate

Fructose-1,6-diphosphate can be converted to glucose-1-phosphate by three successive reactions: Fructose 1,6 diphosphate + H20 ---> fructose-6-phosphate + Pi Keq= 607 Fructose-6-phosphate ---> glucose-6-phosphate G°= -410 cal/mol Glucose-6-phosphate --->glucose-1-phosphate G°= 1750 cal/mol Assu ...continues

The Keq of the reaction below is 1.0.

The Keq of the reaction below is 1.0. What is the G′ under steady state conditions where G-1-P = 10^-5 M, ATP = 2 x 10^3 M, ADPG= 10^-3 M, and PPi = 10^-3. Also, which direction will the reaction proceed under spontaneous conditions, and why?? glucose-1-phosphate + ATP _____> adenosine-diphosphate-glucose + PPi