Biochemistry questions

Please verify if my answers are correct. If not, please correct them. PLEASE only answer if you are confident in your answers. 1. e 2. e 3.b 4.d

You characterize a new enzyme's kinetics.

You characterize a new enzyme's kinetics. Using a saturating (very high so all E is ES) substrate concentration, you measure the initial rate of the reaction at various enzyme concentrations. Does this data fit the Michaelis Menton model/equation? If not, what may be happening? E (nM), rate (mM,s) 5, 50 10, 225 15 ...continues

Biochemistry Questions

Please see the attached questions. I have already answered to the best of my ability. Please check my answers, and if incorrect, please correct them. Thanks! 1.TRUE 2.FALSE 3.TRUE 4. FALSE 5.FALSE

Lineweaver-Burk Plot from Absorbance Data (with and without inhibitor).

I carried out a practical last week and need to write it up but canot remember how to do the calculations from the Michaelis Menten graph plotted which I guess helps me to plot a Lineweaverburk. I have attached the practical requirements. I have to produce a table (attached) but cannot fill in certain columns until I can work ...continues

Beta-Oxidation of Odd and Even Carbon Fatty Acids

1 BCH-100 Fall 2007 BLH #13 (4 points) Question #1: (0.8 point) How many acetyl CoA’s will come from a 12 carbon acyl-CoA? a.) 5 b.) 6 c.) 7 d.) 12 e.) none Question #2: (0.8 point) How many acetyl CoA’s will come from a 13 carbon acyl-CoA? a.) 5 b.) 6 c.) 7 d.) 12 e.) none Question #3: (0.8 point) How many p ...continues

How to calculate the transformation efficiency of competent bacterial cells.

I need help with this transformation efficiency problem: You add 10 ng of pGEM to 50 ul of competent cells, and then dilute this into a total of 1 ml Luria broth after the transformation. You take 100 ul of the Luria broth and plate on and agar plate. The next day, there are 780 colonies on the plate. What was the transfo ...continues

Biochemistry - Which of the following is NOT true about the light reactions of photosynthesis?

Please see the following questions. If I have answered incorrectly, please correct them. Thanks for the help! 1.E 2.e 3.c 4.c 5.b Question #1: (0. 8 point) Which of the following is NOT true about the light reactions of photosynthesis? a.) there are two photosystems linked by an electron transport chain b.) electron ...continues

Free Energy for Transport of Cl- Using Membrane Potential

Blood contains 0.1 M Cl-. Brain tissue contains about 0.02 M Cl-. Calculate: (1) the G for the transport of Cl- from blood into brain cells (2) the energy expended by brain cells in transporting Cl- outward against the concentration gradient. Assume a membrane potential of -75mV (inside).

Calculation of pH Gradient Required to Convert ADP to ATP under Non-Standard Conditions

Calculate the pH gradient across the inner mitochondrial membrane that is necessary to drive the ATPase reaction in the direction of ATP synthesis under steady state conditions at 25°C where ATP=10^-5 M ADP= 10^-3 M Pi=2 x 10^-2 M Also- do not ignore the effect of the membrane potential!

Na/Kpump

Problem: How much energy is needed for the Na/K pump to move 3 Na out and 2 K in when Nai (Na inside)= 9.2mM, Nao (outside)= 115, Ki (K inside)= 135mM and Ko (K outside)= 3mM. Include the effects of membrane potential; it will have to be calculated based on the given ion concentrations and PNa/Pk of 0.013.