Two duplex (ie. double stranded) DNA fragments are shown below. Under identical conditions (ie. ionic strength, buffer composition, temperature, etc.), which double-stranded fragment would denature at the lower temperature? Fragment A: 5' A-A-G-T-A-G-T-T-T-C-A 3' 3' T-T-C-A-T-C-A-A-A-G-T 5' Fragment B: 5' G-C-A-G-G ...continues
A metabolic pathway is represented below. Each step is catalyzed by a different enzyme. (please refer to the attached file to see the reactions within the pathway) (a) Which step would be inhibited by the accumulation of the product E? (b) Which step would be inhibited by the accumulation of the product H ...continues
Classifying different molecules as carbohydrates, lipids, nucleic acids or proteins.
Identify each of the following substances as a carbohydrate, lipid, nucleic acid, protein, or "none of these". (a) antibody (b) actin (c) fructose (d) cellulose (e) RNA (f) beta-galactosidase (g) heme (h) phospholipase A2 (i) glucose (j) tryptophan (k) glycogen (l) cholesterol (m) hemoglobin
How do you calculate specific activity, total activity, and % radioactivity given the information in the attachment?
An overview of glycolysis, the TCA cycle, and the electron transport chain is included.
I Ddescribe the biochemical pathways in the aerobic respiration of glucose and where each takes place within the cell.
Calculating the productivity of a continuous culture given MUmax.
A batch animal cell culture has a productivity of 1.8 g/L*day, with a MUmax=0.5 days, a 10% inoculums and a turnaround time of 5 hours. If a continuous culture could operate at a dilution rate of 90% MUmax, what would be the productivity?
Nutrient environment and its effect on the Lac operon.
What would be the effect on lac operon expression in the following media that contains a strain of E.Coli? What if this mutant also contained an O^- mutation? a. Glucose b. Lactose c. Glucose + Lactose
Constitutive expression of the trp (Tryptophan) operon is examined.
A mutant strain of E.coli has constitutive expression of the trp operon. How could this mutant phenotype result? Ideas are explained.
Understanding how trp operon mRNA is present but no tryptophan.
A mutant strain of E.coli expresses trp operon mRNA but still cannot synthesize trypophan. How could this mutant phenotype result?
In a normal diploid cell, polyploidy can be induced by treatment with colchicine. The drug must exert its effect during which phase of the cell growth or division?
