Diluting

1)a 50ml stock solution of pigelase has a concentration o 10g/ml. How could you make a solution that contained only 0.01g/ml of pigelase. in a final note you cannot use more than 1 ml of water 2) a solution of DNA has a concentration of 2mg/ml. 20 microliter of a 10^-2 (ten to the negative two) dilution of the original sol ...continues

diluting

140 microliters (ul)of a solution containing o.1M NaCl and 60micrograms/ml of DNA is mixed with 50ul of a solution containing 0.02M NaCl and 50ul/ml of DNA. what is the concentration of NaCl and DNA in the mixture? I dont know what to do, i know i should know this by now please help me.

Diluting

1) a biological test pond has a volume of 2500 L and a sulfate concentration of 0.002mM. Two liters of 3M sulfuric acid are added to pond. What is the concentration of sulfate in the test pond? 2) a solution contains the protein insulin at a concentration of 5 mg/ml. You wish to transfer 2 microliters of insulin to anoth ...continues

Chromosome number in different body cells

An animal cell has 46 post-mitotic chromosomes in each of its somatic cells. How many chromosomes should be present in the following cells- a) Mature egg, b) 1st polar body, c) Sperm, d) Spermatid, e) Primary spermatocyte, f) Brain cell, g) Secondary oocyte, h) Spermatogonium.

Complimentary sequence prediction

A strand of DNA is found to have the sequence 5′ AACGTACGTAC 3′. What is the sequence of the nucleotides on the 3′, 5′ strand? Determine the sequence of the m-RNA template of the given sequence.

Triplet codons and probability

Find the number of triplet codons feasible from 4 ribonucleotides A, U, G and C containing a) no uracil, b) one or more uracils.

Predicting triplets in laboratory conditions

Under laboratory conditions, a synthetic polyribonucleiotide was produced using a mixture of A and G with a relative frequency of 5:1 respectively. How would a scientist predict the relative frequencies in which the triplets are expected to form when the ribonucleotides form in a random linear array? Indicate which amino acid(s) ...continues

Probability in drosophila mutation experiment

In a mutation experiment in drosophila, 45 mutations were detected amongst 543 males that received 2500 Roentgens (R) and 67 mutations in 649 males that received 4000R. What is the number of progeny expected in 1000 progeny of males exposed to 6000R?

Prediction of DNA strandedness using base composition

In one set of Φ X phages the deoxyribonucleotide composition was A = 24.7, T = 32.7, G = 24.1 and C = 18.5. Another set of phages had the following deoxyribonucleotide composition - A = 27.6, T = 27.8, G = 22.3 and C = 22.3. a) Find the A/T, G/C ratio of the 2nd set of phages. b) What is the structure of the phage DNA wit ...continues

Composition of bases in yeast

DNA of a certain species of yeast has thymidilic acid content of 32.6%. If the A/T ratio is 0.97, what is the composition of deoxyadenylic acid?