If you spread 0.1 ml of 10-3 of a sewage sample on a plate, and obtained
221 colonies on the plate after incubation, how many CFU/ml were present
in the undiluted sewage sample? Please show work
How can you make E.coli cells competent so that they can be transformed?
What is the appearance of transformed bacterial colonies?
After transformation procedure, your test tube may contain both
transformed and nontransformed cells.
which will grow on LB plate? Which will grow on LB/amp plates?
(+ DNA or – DNA)
Why is there heavy growth on LB and very little growth on LB/amp?
What is the reason to plate E.coli cells on LB/amp?
Why are there both green and white colonies on LB/amp?
Biology Homework Solutions
#120762
Microbiology Questions related to transformation and competent cells
1. If you spread 0.1 ml of 10-3 of a sewage sample on a plate, and obtained 221 colonies on the plate after incubation, how many CFU/ml were present in the undiluted sewage sample? Please show work
2. How can you make E.coli cells competent so that they can be transformed?
3. What is the appearance of transformed bacterial colonies?
4. After transformation procedure, your test tube may contain both transformed and nontransformed cells.
a. which will grow on LB plate? Which will grow on LB/amp plates?
(+ DNA or – DNA)
5 Why is there heavy growth on LB and very little growth on LB/amp?
5. What is the reason to plate E.coli cells on LB/amp?
6. Why are there both green and white colonies on LB/amp?
E.coli cells can be made competent by calcium chloride treatment.
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