Vectors - Briefly describe the purpose of the following features found in all or many plasmid cloning vectors.
a) origin of replication (ori)
b) multiple cloning site (polylinker)
c) antibiotic resistane g ...
Transformation - What is the expected result for the following:
One control sample of competent cells without plasmid was placed on an agar plate without ampicillin. The other consisted of plating cells without pla ...
Plasmid mapping - A plasmid digest with BamHI produced 2 kb and 4 kb fragments. Digest of a plasmid with HindIII produced 6 kb fragment. Double digest with both enzymes produced 1 kb, 2 kb and 3 kb fragments. Draw a ...