You have decided to determine how many microbes are living on the lettuce in the salad bar at your favorite restaurant. You place 1 gram of lettuce and 99 mls of water in a blender and blend the mixture. This is sample A. You then transfer 1 ml of this dilution into to another that contains 9 mls of water. This becomes sampl ...continues
Mutations affecting the function of lac operon
Mutations can alter the function of an operon. Predict how the following mutations would affect the function of the lac operon in the presence and absence of allolactose (lactose). Please indicate if transcription will occur or if transcription will not occur. If this result is the same as what would have occured without the ...continues
If all the fungi were removed from our Pacific Northwest Forests how would that change our forests? (brief but thorough)
What is a genus of archae that are found in human intestines? What other (doesn't happen in the intestines) very useful process do these organisms also help us with?
Can you please confirm my choices for the below question? 1. A denatured protein is one that a. Has lost its three dimensional structure. b. Has reverted to a carbohydrate. c. Lacks amino acids. d. Lacks covalent bonds. My choice is (D) 2. Ions and atoms of an element differ in the a. Number of electrons they posse ...continues
The combination of atoms in molecules
The combination of atoms in molecules depends in large measure on the a) Number of neutrons b) Placement of electrons in the shells c) Presence of organic matter in the environment d) Proton configuration of the nucleus
Can you please confirm my choices for the 8 questions listed below. I was unable to make any choice for one or two of the questions, any help will be appreciated. 1. All of the following characteristics apply to the prokaryotes except a. The lack a nucleus. b. The reproduce only by mitosis. c. They are composed of singl ...continues
I give you a very expensive strain of E. coli containing 10^12 CFU/ml and tell you that I need exactly 10ml of the same E. coli culture at a concentration of 10^4 CFU/ml (I don’t want any excess culture). Using a dilution series, how would you get the correct concentration and volume of bacteria? Make this a realistic scenario.
Perform a 2-fold serial dilution using 4 tubes containing 1ml of NB each and a 20ml broth culture of Bacillus subtilis; use the same B. subtilis culture and perform a 2-fold serial dilution using 4 tubes containing 2ml of NB each; repeat in a similar fashion using 4 tubes containing 0.5ml each.
I am looking for a detailed solution and explanation. I give you 10mls of an overnight culture of Micrococcus luteus at a concentration of 4.7 x 10^7 CFU/ml. Diagram a dilution series by using tubes containing 9ml nutrient broth (NB) and fixed-volume 1ml pipettes (won’t measure any more or less than 1ml).
