Question 1
According to Chargaff's rule, which of the following base-pairings is correct?
a. Adenine—guanine.
b. Guanine – uracil.
c. Thymine – cytosine.
d. Adenine –thymine.
Question 2
How do mutations affect an organism?
a. They may cause the development of a disease-causing allele.
b. They may cause the development of a more beneficial allele.
c. They, in some cases, may have no noticeable affect.
d. They do everything listed above.
Question 3
Genes provide the information needed to produce _____ in the cell.
a. Ribosomes.
b. Enzymes.
c. Lipids.
d. Glucose.
Question 4
Which of the following would indicate a base pairing mutation in DNA?
a. An A paired with a T.
b. A C paired with a G.
c. A G paired with a T.
d. All listed above are improper base pairs.
Question 5
How many enzymes are typically produced from a single gene?
a. One.
b. Two.
c. Three.
d. An unlimited number.
Question 6
Which of the following can produce sticky ends in DNA sequences?
a. Plasmids.
b. Transformation.
c. Restriction enzymes.
d. Bacteria.
Question 7
Two different restriction enzymes recognize:
a. Two different DNA sequences.
b. Two different plasmids.
c. Two different types of bacteria.
d. Two different human genes.
Question 8
You are attempting to insert a human gene into a plasmid. You cut the plasmid with a certain restriction enzyme and you cut the human gene with a different restriction enzyme. You find the sticky ends produced in the plasmid are not complementary to those in the human gene. What happened?
a. There must have been a mutation.
b. The restriction enzymes must have made a mistake when they cut the DNA.
c. The two different restriction enzymes used had different DNA recognition sequences.
d. There must have not been a mutation.
Question 9
In a PCR reaction, the strands of DNA are first separated by ___.
a. Heating.
b. Adding alcohol.
c. Treatment with an acid.
d. Treatment with a strong base.
Question 10
The process by which one learns about the order of bases in a segment of DNA is called ___.
a. The polymerase chain reaction.
b. Sequencing.
c. Cloning.
d. Regulation.