Given: 1. HPLC instrument/ 2. C18 reverse phase column/ 3. 1mM solution of each of the following compounds in Methanol/ 4. Mobile phase is methanol-buffer mixture:
Compounds are 3 seperate groups:
1. Carboxylic Acids:
a. Benzoic acid b.Phthalic acid c. Toluic acid
2. Amino:
a. aniline b.4-aminotoluene c. orhto-
pheylenediamine
3.Amino Carboylic Acids:
a. 4-aminobenzoic acid b. 2-aminobenzoic acid
c.4-amino-2-methylbenzoic acid
I need a suggestion for the buffer and mobile phase composition to get the best separation.