Chemistry Homework Solutions
Problem
#25367

pH and Biochemistry

I need a little nudge to get me started:

Determine the weight in grams of sodium dihydrogen phosphate (M.W.138.01) and disodium hydrogen phosphate (M.W 141.98) needed to  preparte 1L if a standard buffer at pH 7.00 with a total phosphate concentration of 0.100M.

Okay I know that the pKa is going to be 6.86.  I am therefore going to take the difference of pH 7 and pH 6.86 to get 0.14.  I then do 10^14 to get 1.38.  But them I am lost after that.  I can't seem to figure out the concentration.  Once I have that I know that I can find the mass of each of the molecules.

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Question.doc
Determine the weight in grams of sodium dihydrogen phosphate
(M.W.138.01) and disodium hydrogen phosphate (M.W 141.98) needed to
preparte 1L if a standard buffer at pH 7.00 with a total phosphate
concentration of 0.100M.

Okay I know that the pKa is going to be 6.86. I am therefore going to
take the difference of pH 7 and pH 6.86 to get 0.14. I then do 10^14 to
get 1.38. But them I am lost after that. I can’t seem to figure out
the concentration. Once I have that I know that I can find the mass of
each of the molecules.
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