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Spectrophotometry, Absorbance and Transmittance

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1.A portable photometer with a linear response to radiation registered 73.6 μA with a blank solution in the light path. Replacement of the blank with an absorbing solution yielded a response of 24.9 μA. Calculate:

a) The percent transmittance of the sample solution.

b) The absorbance of the sample solution.

c) The expected transmittance if the concentration of absorber is one third that of the original solution.

d) The expected transmittance if the concentration of absorber is double that of the original solution.

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Solution Summary

This solution explains:

1) How to calculate percent transmittance given a linear response spectrophotometer and two measurements.

2) How to convert from percent transmittance to absorbance.

3) How a decrease in absorber in a sample will affect transmittance.

4) How an increase in absorber in a sample will affect transmittance.

Solution Preview

.A portable photometer with a linear response to radiation registered 73.6 μA with a blank solution in the light path. Replacement of the blank with an absorbing solution yielded a response of 24.9 μA. Calculate:

a) The percent transmittance of the sample solution.

If the response is linear, you can set up a proportion with the blank as 100% transmittance:

b) The ...

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