Transcription, splicing and translation
Not what you're looking for?
The file called "genetics2" contains the original assignment with the background information and questions to be solved. Files Unit+4+notes and Unit+5+notes contain more extensive background information, which is not directly related to the assignment. The attached file 407374Instrucations contains the original questions and advise on answering these questions (in blue ink). This solution is also pasted as a simple text here.
Purchase this Solution
Solution Summary
The solution addresses nine questions covering the process of transcription, splicing and translation of the human beta-globin gene. First, the solution discusses the meaning and the function of a promoter in transcription initiation. Then, we talk about the generation of the mRNA transcript, and the splicing process. Next, we cover the translation initiation and the importance of specific amino acids for the protein function. We learn how to work with the sequence provided in GenBank to identify the coding sequence of the gene.
Solution Preview
III) Please answer the following questions. IMPORTANT: When referring to specific
nucleotides, give the letters of the bases (A,C,G or T), as well as their numerical positions in the original DNA sequence. For questions 1-6, please just give me the bases and their numbers, or the range of bases--don't include the question or add any editorial material. (Ex."GAC at 234, 235, 236" or "pre-mRNA = 456-879" if I am asking for a range of nucleotides).
1. a. How many nucleotides in the entire sequence entry?
Answer: As it is indicated in the introductory part, there are 2052 nucleotides
b. How many exons are in the gene?
Answer: You can see from the scheme there are 3 exons, which are labeled with E1, E2, E3.
c. How many introns are in the gene?
Answer: You can see from the scheme there are 2 introns, which are labeled with I1 and I2
To help you answer later questions, please highlight the three exons.
2. Transcription is initiated by the binding of transcription factors to the promoter region at the front end of the gene. The promoter region usually includes a variable number of nucleotides that lie in front of exon 1, and often includes approximately the first third of exon 1. Transcription factor binding sites sometimes have characteristic sequences. Among these are what people cutely call the "cat box motif," which involves the nucleotides C, A and T in some arrangement that spells out CAT or something like it, examples: CAAT, CCAATT, CATAAAA. Please identify two sequences that might serve as "cat box" promoter sequences. First remind ...
Purchase this Solution
Free BrainMass Quizzes
Vision and Oculomotor Control
This quiz will test the student's knowledge of the neural underpinnings of the visual system and its central pathways.
Identifying Variables in Science Experiments, Part 2
Using sample experiments, test yourself to see if you can identify independent, dependent, and controlled variables. Identifying variables is key in understanding and developing experiments. The questions are biology related, but this can be applied to any area of science.
Pregnancy Knowledge
How much do you know about being pregnant? Test your Pregnancy IQ with this quiz!
Creating a Birth Plan
Preparing for a birth and want to make sure that you're including all the right information? Use this quiz to get on the right track and check your birth plan knowledge!
Bacterial Genetics
This quiz test your knowledge of the genetics of bacteria.