Purchase Solution

Concentration of the Protein in the Serum

Not what you're looking for?

Ask Custom Question

2.00 mL serum sample is analyzed for protein by the modified Kjeldahl method. The sample is digested, the ammonia is distilled into boric acid solution, and 15.0 mL of standard HCl is required for titration of the ammonium borate. The HCl is standardized by treating 0.330g pure (NH3)2SO4 in the same manner. If 33.3 mL acid is required in the standardization titration, what is the concentration of protein in the serum in g% (wt/vol)?

Attachments
Purchase this Solution
Solution Summary

The solution discusses the concentration of protein in the serum based on the Kjedahl method.

Purchase this Solution
Free BrainMass Quizzes
Feeding Babies

Do you know the science behind feeding babies? Test your knowledge with this quiz.

Basic Concepts in Neuroscience

This quiz provides a review of the basic concepts in neuroscience.

Comfort Measures For Labor

Are you ready to doula someone through labor?

Pregnancy Knowledge

How much do you know about being pregnant? Test your Pregnancy IQ with this quiz!

How Much Do You Know About Genetic Inheritance?

Most of us studied the basics of dominant and recessive genes at some point. However, genetics are much more complicated than this. How far beyond the basics does your knowledge go?

View More Free Quizzes
Related BrainMass Solutions

  • concept of bound drug and free drug

    The amount of albumin does not appear to be hugely relavent. In disease states such as sepsis, the serum albumin drops drastically, but the free drug concentration does not appear to increase.

  • Affinity Chromatography

    The decrease in the thickness of the band compared to the serum load sample (well #2) indicates that we lost some of the protein during the process of purification.

  • Specificity of Albumin Binding

    We've added even more Ponceau S to the protein solution. And, even at this high concentration of Ponceau S, BSA can still accept more binding of the Ponceau S.

  • Westen Blot Technique

    The Borrelia cell surface proteins are also what would be considered the "protein of interest" in the above outline for Western Blots I have given.

  • Phosphoric Acid, Iron in Serum, and pH of a Mixture

    Assuming the validity of Beer's Law for the experiment calcualte the concentration of iron in serum..

  • Primary hypokalemic periodic paralysis

    Carbohydrate intake stimulates secretion of insulin, which in turn results in increased uptake of potassium into cells and further reduces the extracellular potassium concentration in the serum.

  • Plotting Absorbance against analyte Concentration

    226499 Spectrophotometry : Plotting Absorbance against analyte Concentration (Regression and Least-Squares Analysis) The data in the table below were obtained during a colorimetric determination of glucose in blood serum.

  • Cell biology multiple choice questions

    In the MAP kinase pathway . p90RSK is phosphorylated by: a. MEK b. MAP kinase c. serum response factor d. RAF Ans. b 10. The discovery of green fluorescent protein (GFP) has greatly facilitated living cell experiments because a.

  • Diagnosis of a disease

    the presence of the patient's antibody in that well.

View More Related Solutions